Ultrastructural and Antigenic Characterization of a Granulocytic Ehrlichiosis Agent Directly Isolated and Stably Cultivated from a Patient in New York State | Zendy

Yasuko Rikihisa | Zendy; Ning Zhi | Zendy; Gary P. Wormser | Zendy; Bei Wen | Zendy; Harold W. Horowitz | Zendy; Karim E. Hechemy | Zendy

Open Access

Ultrastructural and Antigenic Characterization of a Granulocytic Ehrlichiosis Agent Directly Isolated and Stably Cultivated from a Patient in New York State

Author(s) -

Yasuko Rikihisa,

Ning Zhi,

Gary P. Wormser,

Bei Wen,

Harold W. Horowitz,

Karim E. Hechemy

Publication year - 1997

Publication title -

the journal of infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.69

H-Index - 252

eISSN - 1537-6613

pISSN - 0022-1899

DOI - 10.1093/infdis/175.1.210

Subject(s) - ehrlichiosis , ehrlichia , virology , biology , microbiology and biotechnology , ehrlichia chaffeensis , antigen , antiserum , ultrastructure , tick , immunology , anatomy

A human granulocytic ehrlichiosis (HGE) agent with 16S rDNA sequence identical to the published sequence of HGE agents was isolated from a patient from New York State by inoculation of the blood leukocyte fraction directly into a human promyelocytic leukemia cell line HL-60. The HGE agent was also isolated from the leukocyte fraction of the blood and bone marrow of a mouse inoculated with the leukocyte fraction of the patient's blood. The isolate has been passaged in tissue culture 30 times over 8 months. Electron microscopy revealed pleomorphic coccobacilli with a thin and highly rippled outer membrane in the clear inclusion matrix. Comparison of IFA reactivity of antisera obtained from a variety of sources with the cell-cultured HGE agent revealed that 3 HGE agent strains (New York isolate, Wisconsin [BDS] isolate, and a tick-derived isolate) are highly cross-reactive and there are diverse antigenic cross-reactivities between HGE agent and Ehrlichia chaffeensis.

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