Open AccessEffects of Recombinant Murine (rm) Interleukin-12 and rm Interferon- in Mice Infected with Bordetella pertussis
Author(s) -
D. Torre,
Filippo Speranza,
Andrea Pugliese,
L. Perversi,
P. Marone,
Giulio Ferrario
Publication year - 1996
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1093/infdis/174.4.786
Subject(s) - bordetella pertussis , recombinant dna , pertussis vaccine , microbiology and biotechnology , pertussis toxin , interferon gamma , interferon , whooping cough , biology , immunology , cytokine , virology , bacteria , immune system , receptor , vaccination , immunization , g protein , gene , biochemistry , genetics
The recombinant cytokines interferon (IFN)-gamma and interleukin (IL)-12 stimulate several macrophage-mediated functions that are important in host defense. An experimental pertussis model showed that intraperitoneal (i.p.) administration of 10,000 U of recombinant murine (rm) IFN-gamma to mice at the time of Bordetella pertussis infection caused a marked and significant reduction in the number of colony-forming units of bacteria in the lungs. Administration i.p. of 1 microgram of rmIL-12 or 1 microgram of rmIL-12 at the time of and for 5 consecutive days after B. pertussis challenge also induced a significant reduction in the number. However, i.p. administration of 1 microgram of rmIL-12 with 10,000 U of IFN-gamma at the time of B. pertussis challenge did not provide protection. These findings indicate that exogenous administration of rmIL-12 and rmIFN-gamma enhances resistance of mice to B. pertussis infection.
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