Open AccessA New Model of Experimental Prosthetic Joint Infection Due to Methicillin-Resistant Staphylococcus aureus: A Microbiologic, Histopathologic, and Magnetic Resonance Imaging Characterization
Author(s) -
Nadia Belmatoug,
A Crémieux,
R. Bléton,
Andreas Volk,
Azzam SalehMghir,
M Grossin,
Louis Garry,
C Carbon
Publication year - 1996
Publication title -
the journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.69
H-Index - 252
eISSN - 1537-6613
pISSN - 0022-1899
DOI - 10.1093/infdis/174.2.414
Subject(s) - staphylococcus aureus , magnetic resonance imaging , medicine , staphylococcal infections , bone infection , osteomyelitis , implant , prosthesis , methicillin resistant staphylococcus aureus , silicone , pathology , surgery , biology , radiology , materials science , bacteria , composite material , genetics
Partial knee arthroplasty was done in rabbits with a silicone-elastomer implant. Immediately after closing the surgical wound, 5 x 10(6) cfu of methicillin-resistant Staphylococcus aureus was injected into the joint. Disease evolution was studied at different stages of infection up to 8 weeks. Prosthetic infection developed in all animals. Gross pathology and histopathologic changes were characteristic of joint and bone infection. Quantitative bacterial counts from infected bone confirmed disease chronicity. The mean number of colony-forming units per gram of bone +/- SD 1 week after infection was 4.84 +/- 0.24 log10 cfu/g and remained stable from week 1 to week 8. Magnetic resonance imaging showed evidence of prosthetic infection as of week 1, while only mild radiologic changes of bone were seen 2 weeks after infection. This model produces a prosthetic infection that is reproducible and close to that of human prosthetic infection.
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