Open AccessCharacterizing the Embryonic Transcriptome of the Snail Ilyanassa
Author(s) -
J. David Lambert,
Xin Yi Chan,
Barbara Spiecker,
Haven C. Sweet
Publication year - 2010
Publication title -
integrative and comparative biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.328
H-Index - 123
eISSN - 1557-7023
pISSN - 1540-7063
DOI - 10.1093/icb/icq121
Subject(s) - biology , contig , transcriptome , snail , expressed sequence tag , gene , cdna library , genetics , embryo , transcription (linguistics) , complementary dna , computational biology , developmental biology , gene expression , evolutionary biology , ecology , genome , linguistics , philosophy
The snail Ilyanassa obsoleta is a useful model for a variety of investigations in the fields of developmental biology, cell biology, larval ecology, ecotoxicology, parasitology, and chemical ecology. To enhance such studies, we have carried out two cDNA sequencing projects to characterize the mRNA transcripts that are present during development of this embryo. These efforts have generated 480 megabases of new sequence, which have been assembled into transcript contigs and represent thousands of newly identified Ilyanassa genes. We identified the orthologs of 182 transcription factors in these data, focusing on families that are likely to be sequence-specific transcriptional regulators. To demonstrate the utility of identifying and examining such transcripts, we describe the expression pattern during organogenesis for IoOnecut, an Ilyanassa ortholog of the HNF6/onecut family of transcription factors.
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