Mouse-Cuterebra Animal Systemic Insecticide Test, 1982

White mice are infested ocularly with 5 newly hatched larvae of Cuterebra fontinella Clark. Two days later, female mice are weighed and treated orally with candidate insecticides usually formulated in Tween 20; male mice have a plastic collar placed around their necks to prevent grooming, and their bodies are dipped into 80-200 ml of an emulsion of the candidate insecticide usually formulated as an EC in xylene (66 parts), Triton X-100 (10 parts), and AI (25 parts). Highest dosages are 100 mg/kg orally and 1% dentially. Four days later, mice are killed and their bodies examined carefully for encapsulated larvae usually found in the inguinal region. Effectiveness is determined by comparing numbers of larvae in treated mice with numbers in untreated mice. Probit analysis is conducted with corrected percent-kill data.