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Twenty-four experimental units of 5 White Leghorn hens, 74 wk old, were arranged in a randomized block design of 4 replicates (A - D) of 6 treatments (0, 5, 10, 15, 20, and 25 ppm Bay Vi-7533 in feed). Birds were managed in 3-tiered wire cages, 1 bird per cage, and manure was collected on paper beneath each tier. The treatments were randomized within each replicate with one restriction: on a given tier in a given replicate, the far unit was a higher dosage than the near unit. This prevented the contamination of low-dosage manure with high-dosage manure during manure collection. The birds were maintained on a standard layer diet (SLD) for 2 wk, then each unit was offered the appropriate treated feed adlibitum for 3 wk. The feed and water systems were then dismantled, cleaned and replaced, and all units were offered untreated feed until the end of the test, 11 Sep 81. Water troughs were cleaned daily. Treated feed was prepared by adding 200 (5 ppm), 400 (10 ppm), 600 (15 ppm), 800 (20 ppm) or 1000 g (25 ppm) of 0.1% (w/w) feed premix of Bay Vi-7533 to 40 Kg of SLD. Data were collected daily from 14 Aug - 11 Sep, inclusive. The manure of all 5 birds in each unit was combined, and a 150 - 200 ml sampled from each unit was collected in a plastic bag, labelled and frozen. Several weeks after the last sample was collected, the manure was thawed for 48 hr, and each sampled was seeded with 30 first-instar house flies. Seeded samples were held in emergence traps and allowed to develop at ambient laboratory temp and RH. After 4 wk, the adult house flies were counted, and each sample was examined for adults which had died in the manure. An ANOVA was contraindicated because of significant replicate and dosage level-replicate interaction effects. Duncan’s multiple range test was used to compare means of dosage levels.

Insect Growth Regulator as a Poultry FeedthrouGh Treatment for House Fly Control, 1981