Toxicities of Topically Applied Insecticides To Douglas-Fur Tussock Moth, 1979

Topical application assessments were performed to determine the toxicities of 13 insecticides to 4th instar Douglas-fir tussock moth. Insects were selected at random from the 8th generation of a laboratory colony. Eight concentrations of each chemical were serially diluted from a concentrated source prepared for each of three replications of each bioassay. Each chemical was applied in reagent grade acetone. Treatment was performed with an ISCO model M microapplicator equipped with a 0.25 cc tuberculin syringe. A dosage rate of 1 jul/100 mg body weight was used to apply the insecticide to the thoracic dorsa of C02-anesthefized larvae weighing 40-90 mg. After treatment, larvae were fed artificial diet. Mortality was tallied 7 days after treatment.