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J. Salvaing*, N. Peynot, M. N. Bedhane, S. Veniel, E. Pellier, C. Boulesteix, N. Beaujean, N. Daniel, and V. Duranthon* UMR BDR, INRA, ENVA, Université Paris Saclay, 78350 Jouy en Josas, France Present address: Laboratoire de Physiologie Cellulaire et Végétale (LPCV), UMR 1417 INRA / UMR 5168 CNRS-CEA-UGA, Bioscience and Biotechnology Institute of Grenoble (BIG), Commissariat à l’Energie Atomique et aux Energies Renouvelables (CEA), 38054 Grenoble Cedex 9, France Present address: Jigjiga University, Ethiopia Present address: Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille Cedex C5, France Present address: INSERM U1208, INRA USC1361 Stem Cell and Brain Research Institute Department of Pluripotent Stem Cells in Mammals, 18 avenue Doyen Lépine, 69675 Bron, France

Assessment of ‘one-step’ versus ‘sequential’ embryo culture conditions through embryonic genome methylation and hydroxymethylation changes