Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes | Zendy

SookYoung Yoon | Zendy; Jin Hee Eum | Zendy; JeongEun Lee | Zendy; H. C. Lee | Zendy; Yongsoo Kim | Zendy; J. E. Han | Zendy; H Won | Zendy; Si Hong Park | Zendy; Sung Han Shim | Zendy; W. S. Lee | Zendy; R.A. Fissore | Zendy; D. R. Lee | Zendy; Tae Ki Yoon | Zendy

Open Access

Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes

Author(s) -

SookYoung Yoon,

Jin Hee Eum,

JeongEun Lee,

H. C. Lee,

Yongsoo Kim,

J. E. Han,

H Won,

Si Hong Park,

Sung Han Shim,

W. S. Lee,

R.A. Fissore,

D. R. Lee,

Tae Ki Yoon

Publication year - 2012

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/des092

Subject(s) - microinjection , oocyte , oocyte activation , recombinant dna , biology , sperm , phospholipase c , microbiology and biotechnology , human fertilization , spermatocyte , intracellular , chemistry , embryo , biochemistry , signal transduction , anatomy , meiosis , genetics , gene

Oocyte activation is a crucial step that comprises the release of the oocyte from meiotic arrest, pronuclear formation and subsequent embryo development. Oocytes are activated by repetitive increases in the intracellular concentration of free Ca(2+), [Ca(2+)](i) oscillations, which are triggered during fertilization by the introduction of the sperm-specific phospholipase C zeta 1 (PLCZ1). Recent studies have shown that sperm from patients lacking expression of PLCZ1 or expressing mutant forms of PLCZ1 fail to induce [Ca(2+)](i) oscillations or oocyte activation. We first purified recombinant human PLCZ1 (hPLCZ1) protein and evaluated its [Ca(2+)](i) oscillation activity in mouse and human oocytes with the view to investigate its application in the clinic for assisted oocytes activation in lieu of chemical agents.

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