Cytoskeletal analysis of human blastocysts by confocal laser scanning microscopy following vitrification
Author(s) -
Katerina Chatzimeletiou,
Ewan E. Morrison,
Yannis Panagiotidis,
Pierre Vanderzwalmen,
Nikos Prapas,
Yannis Prapas,
Basil C. Tarlatzis,
Alan H. Handyside
Publication year - 2011
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/der344
Subject(s) - vitrification , andrology , biology , aneuploidy , microtubule , human fertilization , spindle apparatus , tubulin , cryopreservation , confocal microscopy , confocal , embryo , anatomy , microbiology and biotechnology , chromosome , cell division , genetics , cell , optics , medicine , physics , gene
Vitrification of human blastocysts is being used increasingly to cryopreserve supernumerary embryos following IVF. In this study, we investigate the effects of aseptic vitrification on the cytoskeleton and development of human blastocysts, by analysing survival rates and spindle and chromosome configurations by fluorescence and confocal laser scanning microscopy.
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