Evaluation of correct endogenous reactive oxygen species content for human sperm capacitation and involvement of the NADPH oxidase system | Zendy

Gabriella Donà | Zendy; Cristina Fiore | Zendy; Alessandra Andrisani | Zendy; Guido Ambrosini | Zendy; Anna Maria Brunati | Zendy; Eugenio Ragazzi | Zendy; Decio Armanini | Zendy; Luciana Bordin | Zendy; Giulio Clari | Zendy

Open Access

Evaluation of correct endogenous reactive oxygen species content for human sperm capacitation and involvement of the NADPH oxidase system

Author(s) -

Gabriella Donà,

Cristina Fiore,

Alessandra Andrisani,

Guido Ambrosini,

Anna Maria Brunati,

Eugenio Ragazzi,

Decio Armanini,

Luciana Bordin,

Giulio Clari

Publication year - 2011

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/der321

Subject(s) - capacitation , nadph oxidase , reactive oxygen species , apocynin , sperm , acrosome reaction , endogeny , tyrosine phosphorylation , phosphorylation , microbiology and biotechnology , chemistry , biochemistry , andrology , biology , medicine

Generation of controlled amounts of reactive oxygen species (ROS) and phosphorylation of protein tyrosine residues (Tyr) are two closely related changes involved in sperm capacitation. This study investigated the effect of altered endogenous ROS production on Tyr-phosphorylation (Tyr-P), acrosome reaction (AR) and cell viability during sperm capacitation. The possible origin of the altered ROS production was also evaluated by apocynin (APO) or oligomycin (Oligo) addition.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research