Microarray assessment of methylation in individual mouse blastocyst stage embryos shows that in vitro culture may have widespread genomic effects | Zendy

Katharine M. Wright | Zendy; Lucia Brown | Zendy; Gregory G. Brown | Zendy; Peter R. Casson | Zendy; Stephen Brown | Zendy

Open Access

Microarray assessment of methylation in individual mouse blastocyst stage embryos shows that in vitro culture may have widespread genomic effects

Author(s) -

Katharine M. Wright,

Lucia Brown,

Gregory G. Brown,

Peter R. Casson,

Stephen Brown

Publication year - 2011

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/der201

Subject(s) - dna methylation , biology , blastocyst , methylation , genetics , genomic imprinting , locus (genetics) , embryo , microarray , cpg site , bisulfite sequencing , genomic dna , microbiology and biotechnology , andrology , gene , embryogenesis , gene expression , medicine

Although assisted reproductive technology (ART) is reported to result in abnormal genomic imprinting and/or altered genomic methylation, few if any studies have used high-throughput methods to analyze genomic methylation in ART embryos. We hypothesized that a microarray-based assessment of genomic methylation could be used to reveal differences between ART and normal preimplantation embryos.

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