Dynamic distribution of NuMA and microtubules in human fetal fibroblasts, developing oocytes and somatic cell nuclear transferred embryos | Zendy

Ximing Xu | Zendy; Xunbao Duan | Zendy; Chang Lu | Zendy; Ge Lin | Zendy; G Lu | Zendy

Open Access

Dynamic distribution of NuMA and microtubules in human fetal fibroblasts, developing oocytes and somatic cell nuclear transferred embryos

Author(s) -

Ximing Xu,

Xunbao Duan,

Chang Lu,

Ge Lin,

G Lu

Publication year - 2011

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/der067

Subject(s) - somatic cell nuclear transfer , microbiology and biotechnology , somatic cell , biology , enucleation , embryo , mitosis , spindle pole body , microtubule , meiosis , zygote , spindle apparatus , andrology , cell division , embryogenesis , genetics , cell , blastocyst , medicine , gene

The nuclear mitotic apparatus (NuMA) plays a central role in the assembly and maintenance of spindle poles. Somatic cell nuclear transfer (SCNT) studies on non-human primates have shown that meiotic spindle removal during enucleation causes depletion of NuMA and the minus-end-directed motor protein (HSET) from the ooplasm, and this in turn leads to failure of embryo development. To determine whether NuMA from somatic cells could compensate for NuMA loss during enucleation, the distribution of NuMA and microtubule organization were investigated in human fibroblasts, developing oocytes and SCNT embryos.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research