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Secondary follicle growth and oocyte maturation during encapsulated three-dimensional culture in rhesus monkeys: effects of gonadotrophins, oxygen and fetuin
Author(s) -
Jing Xu,
Maralee S. Lawson,
Richard R. Yeoman,
K.Y. Francis Pau,
Susan L. Barrett,
Mary B. Zelinski,
Richard L. Stouffer
Publication year - 2011
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/der049
Subject(s) - fetuin , oocyte , follicle , andrology , biology , endocrinology , medicine , embryo , microbiology and biotechnology , glycoprotein
An alginate-based matrix supports the three-dimensional (3D) architecture of non-human primate follicles and, in the presence of FSH, permits the in vitro development of pre-antral follicles to the small antral stage, including the production of ovarian steroids and paracrine factors. The current study investigated the ability of gonadotrophins, fetuin and oxygen (O₂) to improve primate follicle growth and oocyte maturation in vitro.

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