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production and expression of cytochrome P450 aromatase enzyme mRNA in long-term serum-free cultures of pig granulosa cells. J Reprod Fertil 1999b;115:67–77. Shores EM, Picton HM, Hunter MG. Differential regulation of pig theca cell steroidogenesis by LH, insulin-like growth factor I and granulosa cells in serum-free culture. J Reprod Fertil 2000;118:211–219. Soleimani R, De Vos W, Van Oostveldt P, Lierman S, Van Den Broecke R, De Sutter P, Dhont M, Van Der Elst J. Two novel techniques to detect follicles in human ovarian cortical tissue. Hum Reprod 2006; 21:1720–1724. Soleimani R, Heytens E, Van den Broecke R, Rottiers I, Dhont M, Cuvelier CA, De Sutter P. Xenotransplantation of cryopreserved human ovarian tissue into murine back muscle. Hum Reprod 2010; 25:1458–1470. Zuang V The neutral red release assay: A review. Altern Lab Anim 2001; 29:575–599.

Constitutional change of Italian legislation on assisted reproduction technology improves pregnancy rate especially in older patients