Morphometric dimensions of the human sperm head depend on the staining method used | Zendy

Liana Maree | Zendy; Stefan S. du Plessis | Zendy; Roelof Menkveld | Zendy; Gerhard van der Horst | Zendy

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Morphometric dimensions of the human sperm head depend on the staining method used

Author(s) -

Liana Maree,

Stefan S. du Plessis,

Roelof Menkveld,

Gerhard van der Horst

Publication year - 2010

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/deq075

Subject(s) - sperm , semen , staining , andrology , biology , papanicolaou stain , population , stain , anatomy , medicine , genetics , cervical cancer , environmental health , cancer

Assessment of sperm morphology (including morphometry) is extensively used to determine one of the qualities of a semen sample and depends on the differential staining of spermatozoa. A staining technique should cause as little change to sperm dimensions and form as possible in order to reliably evaluate the morphometric features of the sperm. Various staining techniques have been employed, but only a few have been recommended by the World Health Organization and are amenable to automated sperm morphometry analysis. Our study was aimed at comparing the effect of three staining techniques [Papanicolaou (PAP), Rapidiff (RD) and SpermBlue (SB)] on human sperm head dimensions and to compare these with the head dimensions in fresh semen.

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