Character, distribution and biological implications of ice crystallization in cryopreserved rabbit ovarian tissue revealed by cryo-scanning electron microscopy | Zendy

Roger G. Gosden | Zendy; Hang Yin | Zendy; Richard Bodine | Zendy; G.J. Morris | Zendy

Open Access

Character, distribution and biological implications of ice crystallization in cryopreserved rabbit ovarian tissue revealed by cryo-scanning electron microscopy

Author(s) -

Roger G. Gosden,

Hang Yin,

Richard Bodine,

G.J. Morris

Publication year - 2009

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/dep395

Subject(s) - cryopreservation , ovarian tissue , scanning electron microscope , crystallization , microscopy , biological tissue , transplantation , biomedical engineering , biology , electron microscope , andrology , pathology , materials science , microbiology and biotechnology , embryo , chemistry , ovary , medicine , surgery , optics , endocrinology , physics , organic chemistry , composite material

Ovarian tissue banking is an emerging strategy for fertility preservation which has led to several viable pregnancies after transplantation. However, the standard method of slow cooling was never rigorously optimized for human tissue nor has the extent and location of ice crystals in tissue been investigated. To address this, we used cryo-scanning electron microscopy (cryo-SEM) to study ice formation in cryopreserved ovarian tissue.

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