Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome | Zendy

M. Kheirollahi-Kouhestani | Zendy; Shahnaz Razavi | Zendy; Marziyeh Tavalaee | Zendy; Mohammad Reza Deemeh | Zendy; Mohammad Mardani | Zendy; Jamal Moshtaghian | Zendy; Mohammad Hossein NasrEsfahani | Zendy

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Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome

Author(s) -

M. Kheirollahi-Kouhestani,

Shahnaz Razavi,

Marziyeh Tavalaee,

Mohammad Reza Deemeh,

Mohammad Mardani,

Jamal Moshtaghian,

Mohammad Hossein NasrEsfahani

Publication year - 2009

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/dep088

Subject(s) - sperm , dna fragmentation , andrology , human fertilization , insemination , semen , pregnancy rate , acridine orange , sperm washing , biology , protamine , pregnancy , medicine , genetics , staining , biochemistry , apoptosis , heparin , programmed cell death

Reducing the percentage of sperm anomalies in insemination samples remains a goal to be achieved in the intra-cytoplasmic sperm insemination (ICSI) procedure. The aim of this study was to evaluate the efficiency of density gradient centrifugation (DGC) and Zeta methods to recover sperm with intact chromatin, and to evaluate whether the combined DGC/Zeta procedure improved ICSI outcome.

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