Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor | Zendy

Raquel MartínIbáñez | Zendy; Christian Unger | Zendy; Anna Strömberg | Zendy; Duncan Baker | Zendy; Josep M. Canals | Zendy; Outi Hovatta | Zendy

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Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor

Author(s) -

Raquel MartínIbáñez,

Christian Unger,

Anna Strömberg,

Duncan Baker,

Josep M. Canals,

Outi Hovatta

Publication year - 2008

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/den316

Subject(s) - cryopreservation , embryonic stem cell , embryoid body , biology , microbiology and biotechnology , regenerative medicine , vitrification , germ layer , stem cell , induced pluripotent stem cell , andrology , embryo , biochemistry , medicine , gene

Human embryonic stem cells (hESCs) have potential use in clinical therapy and regenerative medicine. One of the major challenges regarding the application of these cells is the development of an efficient cryopreservation protocol, since current methods, which include slow-freezing-rapid thawing and vitrification of colonies in suspension, present poor viability and high differentiation rates. Dissociated hESC suspensions do not survive cryopreservation because they are susceptible to apoptosis upon cell detachment and dissociation. A selective Rho-associated kinase (ROCK) inhibitor has been reported to increase the survival of dissociated hESCs and their cloning efficiency.

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