Nuclear staining identifies two populations of human sperm with different DNA fragmentation extent and relationship with semen parameters | Zendy

Monica Muratori | Zendy; Sara Marchiani | Zendy; Lara Tamburrino | Zendy; Valentina Tocci | Zendy; Paola Failli | Zendy; Gianni Forti | Zendy; Elisabetta Baldi | Zendy

Open Access

Nuclear staining identifies two populations of human sperm with different DNA fragmentation extent and relationship with semen parameters

Author(s) -

Monica Muratori,

Sara Marchiani,

Lara Tamburrino,

Valentina Tocci,

Paola Failli,

Gianni Forti,

Elisabetta Baldi

Publication year - 2008

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/den058

Subject(s) - dna fragmentation , sperm , biology , andrology , semen , population , sperm motility , propidium iodide , terminal deoxynucleotidyl transferase , semen quality , tunel assay , semen analysis , microbiology and biotechnology , genetics , apoptosis , programmed cell death , medicine , infertility , pregnancy , environmental health

Sperm DNA fragmentation is a possible predictive parameter for male fertility status. The occurrence of M540 bodies in semen of subfertile subjects affects flow cytometric investigations in sperm. We set up a new method to evaluate DNA fragmentation excluding M540 bodies.

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