Bulk vitrification of human embryonic stem cells
Author(s) -
Tao Li,
Canquan Zhou,
C. Liu,
Qingyun Mai,
Guanglun Zhuang
Publication year - 2007
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/dem386
Subject(s) - vitrification , cryopreservation , andrology , embryonic stem cell , biology , stem cell , embryo , microbiology and biotechnology , medicine , genetics , gene
The traditional vitrification method cannot keep up with the increased culture and propagation efficiency required to cryopreserve large quantities of vigorously proliferating human embryonic stem (HES) cells. In this study, we describe a newly invented vitrification carrier for cryopreserving large amount of HES cells and evaluate whether this bulk vitrification (BV) method is as effective as the popular open-pulled straw (OPS) vitrification method.
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