Preimplantation development of mouse oocytes activated by different levels of human phospholipase C zeta | Zendy

Yang Yu | Zendy; Christopher M. Saunders | Zendy; F. Anthony Lai | Zendy; Karl Swann | Zendy

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Preimplantation development of mouse oocytes activated by different levels of human phospholipase C zeta

Author(s) -

Yang Yu,

Christopher M. Saunders,

F. Anthony Lai,

Karl Swann

Publication year - 2007

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/dem350

Subject(s) - andrology , microbiology and biotechnology , chemistry , oocyte , biology , medicine , embryo

A sperm-specific phospholipase C zeta (PLCzeta) has been shown to trigger Ca(2+) oscillations in mouse and human oocytes and appears to be the sperm factor responsible for activation at fertilization. Previously, complementary RNA (cRNA) injection was used to introduce PLCzeta into oocytes, but it was unclear how much PLCzeta protein is required for development. Here we have injected cRNA encoding luciferase-tagged human PLCzeta (hPLCzeta-luc) into mouse oocytes and established the relationship between hPLCzeta-luc expression, Ca(2+) oscillations and development.

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