Development of a cryopreservation protocol for Leydig cells | Zendy

Guorong Chen | Zendy; RenShan Ge | Zendy; Han Lin | Zendy; Lei Dong | Zendy; Chantal M. Sottas | Zendy; Matthew P. Hardy | Zendy

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Development of a cryopreservation protocol for Leydig cells

Author(s) -

Guorong Chen,

RenShan Ge,

Han Lin,

Lei Dong,

Chantal M. Sottas,

Matthew P. Hardy

Publication year - 2007

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/dem169

Subject(s) - trypan blue , leydig cell , cryopreservation , andrology , biology , viability assay , progenitor cell , staining , bovine serum albumin , dimethyl sulfoxide , endocrinology , cell , medicine , stem cell , immunology , embryo , chemistry , biochemistry , microbiology and biotechnology , luteinizing hormone , genetics , organic chemistry , hormone

In the present study, we describe a procedure to cryopreserve the postnatal members of the Leydig cell lineage, including progenitor (PLC), immature (ILC) and adult (ALC) Leydig cells from, respectively 21-, 35- and 90-day-old rats.

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