Generation of progeny via ICSI following enrichment of elongated spermatids from mouse testis by flow-cytometric cell sorting
Author(s) -
Hiroshi Ohta,
Yuko Sakaide,
Teruhiko Wakayama
Publication year - 2007
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/dem064
Subject(s) - spermatid , andrology , flow cytometry , population , biology , spermatogenesis , spermiogenesis , cell sorting , phenotype , microbiology and biotechnology , genetics , medicine , gene , environmental health
Although ICSI is a useful technique, low elongated spermatid numbers frequently causes technical difficulties, especially in the case of azoospermic patients. Enrichment of elongated spermatids from the testis prior to ICSI may solve this problem.
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