The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells | Zendy

Salwan AlNasiry | Zendy; Nele Geusens | Zendy; M. Hanssens | Zendy; C. Luyten | Zendy; Robert Pijnenborg | Zendy

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The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells

Author(s) -

Salwan AlNasiry,

Nele Geusens,

M. Hanssens,

C. Luyten,

Robert Pijnenborg

Publication year - 2007

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/dem011

Subject(s) - viability assay , fibronectin , gentamicin protection assay , cell culture , trophoblast , cell migration , microbiology and biotechnology , choriocarcinoma , biology , incubation , cell , chemistry , andrology , biochemistry , medicine , fetus , western blot , pregnancy , genetics , placenta , gene

The current techniques for quantifying trophoblast viability, migration and invasion are mainly limited by the need to sacrifice the cells during the test procedure. In this study, the vital dye AB (AB) was used to quantify cell number and viability of BeWo and JEG-3 choriocarcinoma cells, as well as their migration and invasion through fibronectin-coated filters.

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