Characterization and depletion of leukocytes from cells isolated from the pre-ovulatory ovarian follicle | Zendy

Péter Fedorcsák | Zendy; Melinda Ráki | Zendy; Ritsa Storeng | Zendy

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Characterization and depletion of leukocytes from cells isolated from the pre-ovulatory ovarian follicle

Author(s) -

Péter Fedorcsák,

Melinda Ráki,

Ritsa Storeng

Publication year - 2007

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/del491

Subject(s) - corpus luteum , follicular phase , ovarian follicle , immune system , biology , follicle , population , follicular fluid , ovary , medicine , endocrinology , andrology , microbiology and biotechnology , immunology , oocyte , embryo , environmental health

Cells isolated from the periovulatory ovarian follicle are often used as a model of ovarian steroidogenesis and corpus luteum formation. The follicular fluid-derived cell (FFDC) population is, however, heterogeneous and in addition to granulosa-lutein cells, non-steroidogenic cells are also present. These non-steroidogenic cells, especially the immune cells, may have important biological functions in this model. Here, we describe a method to isolate FFDC, characterize the phenotype of the immune cells and deplete immune cells from FFDC.

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