The efficiency of magnetic-activated cell sorting and fluorescence-activated cell sorting in the decontamination of testicular cell suspensions in cancer patients | Zendy

Mieke Geens | Zendy; H. Van de Velde | Zendy; Gert De Block | Zendy; Ellen Goossens | Zendy; A. Van Steirteghem | Zendy; Herman Tournaye | Zendy

Open Access

The efficiency of magnetic-activated cell sorting and fluorescence-activated cell sorting in the decontamination of testicular cell suspensions in cancer patients

Author(s) -

Mieke Geens,

H. Van de Velde,

Gert De Block,

Ellen Goossens,

A. Van Steirteghem,

Herman Tournaye

Publication year - 2006

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/del418

Subject(s) - cell sorting , transplantation , cell , biology , andrology , immunomagnetic separation , flow cytometry , microbiology and biotechnology , immunology , cancer research , medicine , biochemistry

Before clinical application, the feasibility and safety of autologous testicular stem cell transplantation should be explored. Apart from limitations in their numbers, spermatogonial stem cells may also be contaminated by malignant cells. Therefore, both enrichment and decontamination before transplantation may be necessary. This study aimed at evaluating the decontaminating potential of magnetic-activated cell sorting (MACS) and/or fluorescence-activated cell sorting (FACS) for both murine and human testicular cell suspensions. In the mouse, the effectiveness of the transplantation technique after cell sorting was also assessed.

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