Detection of human immunodeficiency virus-1 RNA and DNA by extractive and in situ PCR in unprocessed semen and seminal fractions isolated by semen-washing procedure | Zendy

T. Persico | Zendy; Valeria Savasi | Zendy; E. Ferrazzi | Zendy; M. Oneta | Zendy; A.E. Semprini | Zendy; Giuseppe Simoni | Zendy

Open Access

Detection of human immunodeficiency virus-1 RNA and DNA by extractive and in situ PCR in unprocessed semen and seminal fractions isolated by semen-washing procedure

Author(s) -

T. Persico,

Valeria Savasi,

E. Ferrazzi,

M. Oneta,

A.E. Semprini,

Giuseppe Simoni

Publication year - 2006

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/del004

Subject(s) - semen , biology , sperm , virology , rna , nested polymerase chain reaction , andrology , polymerase chain reaction , dna , virus , microbiology and biotechnology , gene , medicine , genetics

To determine the presence of human immunodeficiency virus-1 (HIV-1) viral RNA/DNA in whole semen, in properly isolated seminal fractions and in spermatozoa after swim-up, by extractive nested PCR and to compare the detection of HIV DNA by in situ PCR (IS-PCR) with the results of nested PCR.

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