Oxidative phosphorylation and the tricarboxylic acid cycle are essential for normal development of mouse ovarian follicles | Zendy

G. Wycherley | Zendy; M. T. Kane | Zendy; A. C. Hynes | Zendy

Open Access

Oxidative phosphorylation and the tricarboxylic acid cycle are essential for normal development of mouse ovarian follicles

Author(s) -

G. Wycherley,

M. T. Kane,

A. C. Hynes

Publication year - 2005

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/dei132

Subject(s) - oxidative phosphorylation , follicular phase , citric acid cycle , biology , ovarian follicle , follicle , tricarboxylic acid , metabolism , medicine , malonate , endocrinology , andrology , biochemistry

Mouse ovarian follicles are typically grown in upright drops of culture medium. Recently we found that culture of follicles at the medium-gas interface in inverted drops markedly improved follicular development, possibly due to improved access of oxygen to the follicle. In this study, we examined the importance of aerobic energy metabolism for follicle development by culturing mouse follicles (198 6 16.5 initial microm diameter, mean 6 SD) in the presence of phosphorylation and tricarboxylic acid (TCA) cycle inhibitors.

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