Cryopreservation of human embryonic stem cells without the use of a programmable freezer
Author(s) -
Sung Yun Ha,
Byung Chul Jee,
Chang Suk Suh,
Hee Sun Kim,
Sun Kyung Oh,
Seok Hyun Kim,
Shin Yong Moon
Publication year - 2005
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/deh854
Subject(s) - cryoprotectant , cryopreservation , fetal bovine serum , andrology , dimethyl sulfoxide , embryonic stem cell , glycerol , biology , ethylene glycol , stem cell , cell survival , chemistry , immunology , cell , biochemistry , microbiology and biotechnology , embryo , in vitro , medicine , organic chemistry , gene
An effective freezing-thawing technique is crucial for the clinical application of human embryonic stem (ES) cells. The aim of this study was to find an optimal cryopreservation protocol for human ES cells using slow freezing-rapid thawing without a programmable freezer.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom