Open AccessSequential FISH analysis using competitive displacement of labelled peptide nucleic acid probes for eight chromosomes in human blastomeres
Author(s) -
Inge Agerholm,
Søren Ziebe,
Brett Williams,
C. Berg,
Dorthe Crüger,
George B. Petersen,
Steen Kølvraa
Publication year - 2005
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/deh735
Subject(s) - peptide nucleic acid , nucleic acid , hybridization probe , fish <actinopterygii> , fluorescence in situ hybridization , dna , multiple displacement amplification , blastomere , biology , ctd , nucleic acid thermodynamics , microbiology and biotechnology , chemistry , chromosome , biochemistry , embryo , genetics , base sequence , dna extraction , polymerase chain reaction , gene , fishery , embryogenesis , oceanography , geology
The aim was to introduce a new strategy based on peptide nucleic acid (PNA) probes and competitive displacement for using fluorescence in-situ hybridization (FISH) analysis on human blastomeres.