Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers | Zendy

Saeyoung Park | Zendy

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Establishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers

Author(s) -

Saeyoung Park

Publication year - 2004

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/deh102

Subject(s) - embryonic stem cell , andrology , biology , blastocyst , inner cell mass , embryo , immunology , cell culture , stem cell , microbiology and biotechnology , embryogenesis , genetics , medicine , gene

Recently, human embryonic stem (hES) cells have become very important resources for basic research on cell replacement therapy and other medical applications. The purpose of this study was to test whether pluripotent hES cell lines could be successfully derived from frozen-thawed embryos that were destined to be discarded after 5 years in a routine human IVF-embryo transfer programme and whether an STO cell feeder layer can be used for the culture of hES cells.

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