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The purpose of this study was to test the effectiveness of one two-step (A) and two one-step (B1 and B2) vitrification procedures on denuded expanded or hatching rabbit blastocysts held in standard sealed plastic straws as a possible model for human blastocysts. The effect of blastocyst size was also studied on the basis of three size categories (I: diameter &lt;200 micro m; II: diameter 200-299 micro m; III: diameter &gt;/==" BORDER="0"&gt;300 micro m).

Vitrification of zona-free rabbit expanded or hatching blastocysts: a possible model for human blastocysts