A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells | Zendy

Outi Hovatta | Zendy; Milla Mikkola | Zendy; Karin Gertow | Zendy; AnneMarie Strömberg | Zendy; José Inzunza | Zendy; Julius Hreinsson | Zendy; Björn Rozell | Zendy; Elisabeth Blennow | Zendy; Michael Andäng | Zendy; Lars ÄhrlundRichter | Zendy

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A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells

Author(s) -

Outi Hovatta,

Milla Mikkola,

Karin Gertow,

AnneMarie Strömberg,

José Inzunza,

Julius Hreinsson,

Björn Rozell,

Elisabeth Blennow,

Michael Andäng,

Lars ÄhrlundRichter

Publication year - 2003

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/deg290

Subject(s) - foreskin , biology , embryonic stem cell , stem cell , kosr , andrology , cell culture , homeobox protein nanog , microbiology and biotechnology , matrigel , immunology , induced pluripotent stem cell , medicine , genetics , gene

Human embryonic stem (hES) cell lines were first cultured using fetal mouse fibroblasts as feeder cells. To avoid feeders and to reduce the amount of xeno-components, Matrigel- and laminin-coated dishes, and conditioned mouse feeder cell medium have been used, and hES cells have also been cultured on human fetal muscle and skin, and adult Fallopian tube epithelial cells.

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