Somatic and embryonic cell nucleus transfer into intact and enucleated immature mouse oocytes | Zendy

J. Fulka | Zendy

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Somatic and embryonic cell nucleus transfer into intact and enucleated immature mouse oocytes

Author(s) -

J. Fulka

Publication year - 2002

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/17.8.2160

Subject(s) - somatic cell , cytoplast , polar body , biology , oocyte , premature chromosome condensation , microbiology and biotechnology , metaphase , somatic cell nuclear transfer , embryonic stem cell , meiosis , cell division , cytoplasm , cell , genetics , chromosome , embryo , cell cycle , embryogenesis , blastocyst , gene

The aim of our study was to evaluate the possibility of embryonic or somatic cell haploidization after fusion with intact or enucleated immature oocytes which were subsequently cultured in vitro. Embryonic or somatic cell nuclei do not undergo premature chromosome condensation when fused to intact or enucleated immature oocytes whose maturation is prevented by dibutyryl cyclic AMP (dbcAMP). The presence of dbcAMP permits, however, the completion of DNA replication in somatic cell nuclei.

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