Vitrification of mouse oocytes using closed pulled straws (CPS) achieves a high survival and preserves good patterns of meiotic spindles, compared with conventional straws, open pulled straws (OPS) and grids
Author(s) -
SheeUan Chen,
YihRon Lien,
Yichen Cheng,
HsinFu Chen,
HongNerng Ho,
YuShih Yang
Publication year - 2001
Publication title -
human reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.446
H-Index - 226
eISSN - 1460-2350
pISSN - 0268-1161
DOI - 10.1093/humrep/16.11.2350
Subject(s) - vitrification , human fertilization , straw , andrology , incubation , blastocyst , meiosis , biology , embryo , in vitro fertilisation , anatomy , embryogenesis , microbiology and biotechnology , genetics , medicine , biochemistry , agronomy , gene
We modified the loading of pulled straws into a new closed system, called closed pulled straws (CPS) for holding oocytes for vitrification. The morphological survival, dynamics of meiotic spindles, and fertilization in vitro of vitrified oocytes using CPS were compared with conventional straws, open pulled straws (OPS), and grids.
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