Ooplasmic injection of elongating spermatids for the treatment of non- obstructive azoospermia | Zendy

Nikolaos Sofikitis | Zendy; Yasuhisa Yamamoto | Zendy; Ikuo Miyagawa | Zendy; G. Mekras | Zendy; Yasuyuki Mio | Zendy; T. Toda | Zendy; S. Antypas | Zendy; Hiroshi Kawamura | Zendy; N. Kanakas | Zendy; N Antoniou | Zendy; D. Loutradis | Zendy; Themis Mantzavinos | Zendy; K Kalianidis | Zendy; E. Agapitos | Zendy

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Ooplasmic injection of elongating spermatids for the treatment of non- obstructive azoospermia

Author(s) -

Nikolaos Sofikitis,

Yasuhisa Yamamoto,

Ikuo Miyagawa,

G. Mekras,

Yasuyuki Mio,

T. Toda,

S. Antypas,

Hiroshi Kawamura,

N. Kanakas,

N Antoniou,

D. Loutradis,

Themis Mantzavinos,

K Kalianidis,

E. Agapitos

Publication year - 1998

Publication title -

human reproduction

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.446

H-Index - 226

eISSN - 1460-2350

pISSN - 0268-1161

DOI - 10.1093/humrep/13.3.709

Subject(s) - spermatid , andrology , azoospermia , spermatocyte , obstructive azoospermia , embryo , intracytoplasmic sperm injection , biology , spermatogenesis , meiosis , medicine , microbiology and biotechnology , infertility , pregnancy , in vitro fertilisation , genetics , gene

We applied the technique of ooplasmic elongating spermatid injection to the treatment of non-obstructive azoospermia. Mature oocytes were injected with elongating spermatids isolated from testicular biopsy material obtained from 13 non-obstructed azoospermic men. Seventy-three oocytes were successfully injected with elongating spermatids and were then cultured for 36 h. At 13 h post-injection 68 oocytes were found to be activated and 52 of them were fertilized. Forty-one 2- to 4-cell stage embryos developed from normally fertilized oocytes were transferred. At least two embryos were transferred to each female partner. Two pregnancies were achieved. Elongating spermatid injection may have a role in the treatment of non-obstructive azoospermia.

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