Open AccessRecruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
Author(s) -
Fabrício C. Machado,
Leandro T. De La Cruz,
A. A. da Silva,
Manoel Cruz,
Renato A. Mortara,
Maria Cristina RoqueBarreira,
C. V. da Silva
Publication year - 2013
Publication title -
glycobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.757
H-Index - 128
eISSN - 1460-2423
pISSN - 0959-6658
DOI - 10.1093/glycob/cwt097
Subject(s) - trypanosoma cruzi , intracellular , extracellular , microbiology and biotechnology , vacuole , amastigote , intracellular parasite , phagolysosome , biology , cytosol , galectin , galectin 1 , galectin 3 , phagosome , immunology , leishmania , cytoplasm , biochemistry , parasite hosting , world wide web , computer science , enzyme
The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of β-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. In this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. In addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.
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