Mice lacking α1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors | Zendy

Takashi Kudo | Zendy; Takashi Fujii | Zendy; Shiro Ikegami | Zendy; Kaoru Inokuchi | Zendy; Yuko Takayama | Zendy; Yuzuru Ikehara | Zendy; Shoko Nishihara | Zendy; Akira Togayachi | Zendy; Takashi Sato | Zendy; Kouichi Tachibana | Zendy; Shinji Yuasa | Zendy; Hisashi Narimatsu | Zendy

Open Access

Mice lacking α1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors

Author(s) -

Takashi Kudo,

Takashi Fujii,

Shiro Ikegami,

Kaoru Inokuchi,

Yuko Takayama,

Yuzuru Ikehara,

Shoko Nishihara,

Akira Togayachi,

Takashi Sato,

Kouichi Tachibana,

Shinji Yuasa,

Hisashi Narimatsu

Publication year - 2006

Publication title -

glycobiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.757

H-Index - 128

eISSN - 1460-2423

pISSN - 0959-6658

DOI - 10.1093/glycob/cwl047

Subject(s) - basolateral amygdala , glycolipid , neurite , forebrain , glycoprotein , neuroscience , microbiology and biotechnology , amygdala , biology , chemistry , medicine , central nervous system , biochemistry , in vitro

The 3-fucosyl-N-acetyllactosamine [Lewis x (Le(x)), CD15, SSEA-1] carbohydrate structure is expressed on several glycolipids, glycoproteins, and proteoglycans of the nervous system and has been implicated in cell-cell recognition, neurite outgrowth, and neuronal migration during development. To characterize the functional role of Le(x) carbohydrate structure in vivo, we have generated mutant mice that lack alpha1,3-fucosyltransferase IX (Fut9(-/-)). Fut9(-/-) mice were unable to synthesize the Le(x) structure carried on glycoproteins and glycolipids in embryonic and adult brain. However, no obvious pathological differences between wild-type and Fut9(-/-) mice were found in brain. In behavioral tests, Fut9(-/-) mice exhibited increased anxiety-like responses in dark-light preference and in elevated plus maze tests. Immunohistochemical analysis showed that the number of calbindin-positive neurons was decreased in the basolateral amygdala in Fut9(-/-) mice. These observations indicated that the carbohydrates synthesized by Fut9 play critical roles in functional regulations of interneurons in the amygdalar subdivisions and suggested a role for the Le(x) structure in some aspects of emotional behavior in mice.

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