A collection of yeast cellular electron cryotomography data | Zendy

Lu Gan | Zendy; Cai Tong Ng | Zendy; Chen Chen | Zendy; Shujun Cai | Zendy

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A collection of yeast cellular electron cryotomography data

Author(s) -

Lu Gan,

Cai Tong Ng,

Chen Chen,

Shujun Cai

Publication year - 2019

Publication title -

gigascience

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.947

H-Index - 54

ISSN - 2047-217X

DOI - 10.1093/gigascience/giz077

Subject(s) - budding yeast , discoverability , computer science , electron tomography , in situ , saccharomyces cerevisiae , chromatin , upload , computational biology , yeast , nanotechnology , biological system , biology , chemistry , materials science , dna , genetics , scanning transmission electron microscopy , organic chemistry , human–computer interaction , transmission electron microscopy , operating system

Cells are powered by a large set of macromolecular complexes, which work together in a crowded environment. The in situ mechanisms of these complexes are unclear because their 3D distribution, organization, and interactions are largely unknown. Electron cryotomography (cryo-ET) can address these knowledge gaps because it produces cryotomograms-3D images that reveal biological structure at ∼4-nm resolution. Cryo-ET uses no fixation, dehydration, staining, or plastic embedment, so cellular features are visualized in a life-like, frozen-hydrated state. To study chromatin and mitotic machinery in situ, we subjected yeast cells to genetic and chemical perturbations, cryosectioned them, and then imaged the cells by cryo-ET.

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