Open AccessSingle-cell transcriptomic landscape of nucleated cells in umbilical cord blood
Author(s) -
Yi Zhao,
Xiao Li,
Weihua Zhao,
Jingwan Wang,
Jiawei Yu,
Ziyun Wan,
Kai Gao,
Gang Yi,
Wang Xie,
Bingbing Fan,
Qinkai Wu,
Bangwei Chen,
Feng Xie,
Jinghua Wu,
Wei Zhang,
Fang Chen,
Huanming Yang,
Jian Wang,
Xun Xu,
Bin Li,
Shiping Liu,
Yong Hou,
Xiao Liu
Publication year - 2019
Publication title -
gigascience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.947
H-Index - 54
ISSN - 2047-217X
DOI - 10.1093/gigascience/giz047
Subject(s) - transcriptome , biology , immunology , cell , progenitor cell , epigenetics , cell type , umbilical cord , cord blood , single cell analysis , granzyme , stem cell , microbiology and biotechnology , computational biology , gene , gene expression , genetics , immune system , cd8 , perforin
For both pediatric and adult patients, umbilical cord blood (UCB) transplant is a therapeutic option for a variety of hematologic diseases, such as blood cancers, myeloproliferative disorders, genetic diseases, and metabolic disorders. However, the level of cellular heterogeneity and diversity of nucleated cells in UCB has not yet been assessed in an unbiased and systemic fashion. In the present study, nucleated cells from UCB were subjected to single-cell RNA sequencing to simultaneously profile the gene expression signatures of thousands of cells, generating a rich resource for further functional studies. Here, we report the transcriptomes of 17,637 UCB cells, covering 12 major cell types, many of which can be further divided into distinct subpopulations.
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