Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives | Zendy

Noha Al-Otaibi | Zendy; Juliana S. Cassoli | Zendy; Daniel MartinsdeSouza | Zendy; Nigel K.H. Slater | Zendy; Hassan Rahmoune | Zendy

Open Access

Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives

Author(s) -

Noha Al-Otaibi,

Juliana S. Cassoli,

Daniel MartinsdeSouza,

Nigel K.H. Slater,

Hassan Rahmoune

Publication year - 2018

Publication title -

gigascience

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.947

H-Index - 54

ISSN - 2047-217X

DOI - 10.1093/gigascience/giy155

Subject(s) - cryopreservation , proteome , trehalose , chemistry , biochemistry , glutathione , microbiology and biotechnology , biology , enzyme , embryo

Cryopreservation is a routinely used methodology for prolonged storage of viable cells. The use of cryo-protective agents (CPAs) such as dimethylsulfoxide (DMSO), glycerol, or trehalose is paramount to reducing cellular cryo-injury, but their effectiveness is still limited. The current study focuses on establishing and modulating the proteomic and the corresponding biological profiles associated with the cryo-injury of human leukemia (HL-60) cells cryopreserved in DMSO alone or DMSO +/- novel CPAs (e.g., nigerose [Nig] or salidroside [Sal]).

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