Quantitative super-resolution single molecule microscopy dataset of YFP-tagged growth factor receptors | Zendy

Tomáš Lukeš | Zendy; Jakub Pospíšil | Zendy; Karel Fliegel | Zendy; Theo Lasser | Zendy; Guy M. Hagen | Zendy

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Quantitative super-resolution single molecule microscopy dataset of YFP-tagged growth factor receptors

Author(s) -

Tomáš Lukeš,

Jakub Pospíšil,

Karel Fliegel,

Theo Lasser,

Guy M. Hagen

Publication year - 2018

Publication title -

gigascience

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.947

H-Index - 54

ISSN - 2047-217X

DOI - 10.1093/gigascience/giy002

Subject(s) - resolution (logic) , microscopy , receptor , computational biology , molecule , chemistry , biophysics , biology , computer science , microbiology and biotechnology , physics , biochemistry , optics , artificial intelligence , organic chemistry

Super-resolution single molecule localization microscopy (SMLM) is a method for achieving resolution beyond the classical limit in optical microscopes (approx. 200 nm laterally). Yellow fluorescent protein (YFP) has been used for super-resolution single molecule localization microscopy, but less frequently than other fluorescent probes. Working with YFP in SMLM is a challenge because a lower number of photons are emitted per molecule compared with organic dyes, which are more commonly used. Publically available experimental data can facilitate development of new data analysis algorithms.

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