Finding Nemo: hybrid assembly with Oxford Nanopore and Illumina reads greatly improves the clownfish (Amphiprion ocellaris) genome assembly | Zendy

Mun Hua Tan | Zendy; Christopher M. Austin | Zendy; Michael P. Hammer | Zendy; Yin Peng Lee | Zendy; Larry Croft | Zendy; Han Ming Gan | Zendy

Open Access

Finding Nemo: hybrid assembly with Oxford Nanopore and Illumina reads greatly improves the clownfish (Amphiprion ocellaris) genome assembly

Author(s) -

Mun Hua Tan,

Christopher M. Austin,

Michael P. Hammer,

Yin Peng Lee,

Larry Croft,

Han Ming Gan

Publication year - 2018

Publication title -

gigascience

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.947

H-Index - 54

ISSN - 2047-217X

DOI - 10.1093/gigascience/gix137

Subject(s) - genome , biology , sequence assembly , genome size , nanopore sequencing , evolutionary biology , subfamily , illumina dye sequencing , computational biology , genetics , gene , gene expression , transcriptome

Some of the most widely recognized coral reef fishes are clownfish or anemonefish, members of the family Pomacentridae (subfamily: Amphiprioninae). They are popular aquarium species due to their bright colours, adaptability to captivity, and fascinating behavior. Their breeding biology (sequential hermaphrodites) and symbiotic mutualism with sea anemones have attracted much scientific interest. Moreover, there are some curious geographic-based phenotypes that warrant investigation. Leveraging on the advancement in Nanopore long read technology, we report the first hybrid assembly of the clown anemonefish (Amphiprion ocellaris) genome utilizing Illumina and Nanopore reads, further demonstrating the substantial impact of modest long read sequencing data sets on improving genome assembly statistics.

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