The use of Oxford Nanopore native barcoding for complete genome assembly | Zendy

Sion Bayliss | Zendy; Vicky L. Hunt | Zendy; Maho Yokoyama | Zendy; Harry A. Thorpe | Zendy; Edward J. Feil | Zendy

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The use of Oxford Nanopore native barcoding for complete genome assembly

Author(s) -

Sion Bayliss,

Vicky L. Hunt,

Maho Yokoyama,

Harry A. Thorpe,

Edward J. Feil

Publication year - 2017

Publication title -

gigascience

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.947

H-Index - 54

ISSN - 2047-217X

DOI - 10.1093/gigascience/gix001

Subject(s) - nanopore , genome , nanopore sequencing , dna barcoding , sequence assembly , evolutionary biology , computational biology , biology , nanotechnology , genetics , gene , materials science , gene expression , transcriptome

The Oxford Nanopore Technologies MinION(TM) is a mobile DNA sequencer that can produce long read sequences with a short turn-around time. Here we report the first demonstration of single contig genome assembly using Oxford Nanopore native barcoding when applied to a multiplexed library of 12 samples and combined with existing Illumina short read data. This paves the way for the closure of multiple bacterial genomes from a single MinION(TM) sequencing run, given the availability of existing short read data. The strain we used, MHO_001, represents the important community-acquired methicillin-resistant Staphylococcus aureus lineage USA300.

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