Long-read only assembly of Drechmeria coniospora genomes reveals widespread chromosome plasticity and illustrates the limitations of current nanopore methods
Author(s) -
Damien Courtine,
Jan Provazník,
Jérôme Reboul,
Guillaume Blanc,
Vladimı́r Beneš,
Jonathan J. Ewbank
Publication year - 2020
Publication title -
gigascience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.947
H-Index - 54
ISSN - 2047-217X
DOI - 10.1093/gigascience/giaa099
Subject(s) - nanopore sequencing , genome , nanopore , biology , caenorhabditis elegans , computational biology , dna sequencing , sequence assembly , chromosome , genomics , gene , evolutionary biology , genetics , nanotechnology , gene expression , materials science , transcriptome
Long-read sequencing is increasingly being used to determine eukaryotic genomes. We used nanopore technology to generate chromosome-level assemblies for 3 different strains of Drechmeria coniospora, a nematophagous fungus used extensively in the study of innate immunity in Caenorhabditis elegans.
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