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Analyses that use genome assemblies are critically affected by the contiguity, completeness, and accuracy of those assemblies. In recent years single-molecule sequencing techniques generating long-read information have become available and enabled substantial improvement in contig length and genome completeness, especially for large genomes (&gt;100 Mb), although bioinformatic tools for these applications are still limited.

TGS-GapCloser: A fast and accurate gap closer for large genomes with low coverage of error-prone long reads