Expression of Key Regulators of Mitochondrial Biogenesis in Growth Hormone Receptor Knockout (GHRKO) Mice is Enhanced but is Not Further Improved by Other Potential Life-Extending Interventions | Zendy

Adam Gesing | Zendy; Michał M. Masternak | Zendy; Fang Wang | Zendy; Bertrand Joseph | Zendy; Christiaan Leeuwenburgh | Zendy; Reyhan Westbrook | Zendy; Andrzej Lewiński | Zendy; Małgorzata KarbownikLewińska | Zendy; Andrzej Bartke | Zendy

Open Access

Expression of Key Regulators of Mitochondrial Biogenesis in Growth Hormone Receptor Knockout (GHRKO) Mice is Enhanced but is Not Further Improved by Other Potential Life-Extending Interventions

Author(s) -

Adam Gesing,

Michał M. Masternak,

Fang Wang,

Bertrand Joseph,

Christiaan Leeuwenburgh,

Reyhan Westbrook,

Andrzej Lewiński,

Małgorzata KarbownikLewińska,

Andrzej Bartke

Publication year - 2011

Publication title -

the journals of gerontology series a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.134

H-Index - 189

eISSN - 1758-535X

pISSN - 1079-5006

DOI - 10.1093/gerona/glr080

Subject(s) - tfam , mitochondrial biogenesis , ampk , calorie restriction , endocrinology , medicine , sirtuin 1 , biology , nrf1 , peroxisome proliferator activated receptor , sirtuin , adenosine monophosphate , enos , receptor , microbiology and biotechnology , protein kinase a , adenosine , mitochondrion , nitric oxide synthase , nitric oxide , kinase , biochemistry , downregulation and upregulation , gene , acetylation

Mitochondrial biogenesis is essential for cell viability. Growth hormone receptor knockout (GHRKO), calorie restriction, and surgical visceral fat removal constitute experimental interventions to delay aging and increase life span. We examined the expression of known regulators of mitochondriogenesis: peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α), adenosine monophosphate (AMP)-activated protein kinase (AMPK), sirtuin-1 (SIRT-1) and sirtuin-3 (SIRT-3), endothelial nitric oxide synthase (eNOS), nuclear respiratory factor-1, mitochondrial transcription factor A (TFAM), and mitofusin-2 (MFN-2) in the skeletal muscles and hearts of control and calorie-restricted female GHRKO mice and in the kidneys of male GHRKOs after visceral fat removal or sham surgery. Expression of PGC-1α in skeletal muscles, AMPK, SIRT-1, SIRT-3, eNOS, and MFN-2 in the heart and PGC-1α, AMPK, SIRT-3, eNOS, and MFN-2 in kidneys was increased in GHRKO mice but was not affected by calorie restriction or visceral fat removal. GHRKO mice have increased expression of key regulators of mitochondriogenesis, which is not improved further by calorie restriction or visceral fat removal.

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