A COMPARISON OF TWO-POINT, THREE-POINT AND DELETION MAPPING IN THE C CISTRON OF RHIZOBIOPHAGE 16–3, WITH AN EXPLANATION FOR THE RECOMBINATION PATTERN | Zendy

László Orosz | Zendy; Katalin Rostás | Zendy; Rollin D. Hotchkiss | Zendy

Open Access

A COMPARISON OF TWO-POINT, THREE-POINT AND DELETION MAPPING IN THE C CISTRON OF RHIZOBIOPHAGE 16–3, WITH AN EXPLANATION FOR THE RECOMBINATION PATTERN

Author(s) -

László Orosz,

Katalin Rostás,

Rollin D. Hotchkiss

Publication year - 1980

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/94.2.249

Subject(s) - cistron , recombination , biology , genetics , point mutation , loss of heterozygosity , mutant , allele , gene

A general tendency for additivity prevailed in recombination frequencies for two-point fine-structure mapping of 14 mutants in the C cistron of Rhizobium meliloti phage 16-3, with little evidence of any marker effect. Intracistronic three-point mapping indicated that double crossovers are rare. Deletion mapping indicated that the two- and three-point mapping data gave the correct order of the mutations. A high frequency (5 to 8%) of c/c+ heterozygotic phage progenies was observed in standard crosses. This pattern implies formation of a relatively long region of heterozygosity. Together with the results of the three-point tests, it suggests certain properties of the branch migration and resolution steps envisioned in current mechanisms of recombination.

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