ESCHERICHIA COLI K-12 AUXOTROPHS INDUCED BY INSERTION OF THE TRANSPOSABLE ELEMENT Tn5 | Zendy

Karen Joy Shaw | Zendy; Claire M. Berg | Zendy

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ESCHERICHIA COLI K-12 AUXOTROPHS INDUCED BY INSERTION OF THE TRANSPOSABLE ELEMENT Tn5

Author(s) -

Karen Joy Shaw,

Claire M. Berg

Publication year - 1979

Publication title -

genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.792

H-Index - 246

eISSN - 1943-2631

pISSN - 0016-6731

DOI - 10.1093/genetics/92.3.741

Subject(s) - transposable element , auxotrophy , biology , genetics , insertion , escherichia coli , insertion sequence , dna transposable elements , mutant , chromosome , kanamycin , mutation , gene

The siies of insertion of the transposable kanamycin-neomycin resistance determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.

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